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Her eyes have not yet become the pale yellow colour of fully mature eagles, like the male's eyes have. The female will be called Anna and the male Uku.

Thank you for the beautiful names. The rings of Anna serve as an excellent marker for separating the eagles from each other.

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It is not, however, always possible to see the rings. Then other markers will be needed. In some cases mostly out of breeding season an adult eagle other than Uku may visit nest alone. In these cases Ame CU-68 Webcam is useful to have some other markers for identifying the birds.

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Essential biomedical technologies for LMICs must be Ame CU-68 Webcam and sustainable in low-resource settings. Therefore, these technologies must be simple, reasonably mobile, and based on components and expertise readily available in these settings. In addition, in order to serve large populations the technologies must be Ame CU-68 Webcam for Point-of-Care Testing POCTwhich is medical diagnostic testing performed on-site.

These devices have been integrated into several types of transducers to provide new capabilities such as smartphone attachments for stethoscopes [ 678 ], smartphone-based ambulatory blood pressure monitoring [ 891011Ame CU-68 Webcam ], mobile point-of-care ultrasound [ 13 ], or digital microscopy [ 141516171819202122 ]. New assay technologies such as lateral flow, paper-based microfluidics [ 23 ], phone-based colorimetric readers [ 24 ], or lab-on-a-chip Ame CU-68 Webcam were used for the development of sensitive, low-cost biological assays [ 25 ].

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These include various chemical [ 2627 ] and biological assays for DNA amplification outside of laboratory environments [ 28 ] and analysis of toxin activities and immunological assays for many types of medical settings [ 2930 ]. Optical Detection and Analysis Many biological diagnostic techniques use optical methods for research, clinical, and industrial analysis, including light absorbance, fluorescence, polarization colorimetry, spectrometry, and luminescence [ 31 ].

Several types of optical detectors Ame CU-68 Webcam used for biodetection. For applications requiring Ame CU-68 Webcam light measurements, such as plate readers for flow cytometry, avalanche photodiodes [ 32333435 ] and photomultipliers [ 363738394041 ] are used.

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However, these are Ame CU-68 Webcam detectors that are spatially limited and therefore very slow when analyzing large sample volumes. Optical-based detection is especially attractive for mobile POCT due to its simplicity, size, sensitivity, and cost. Many consumer electronics and wireless telecommunications employ optical detectors [ 49 ], including smartphones, tablets, webcams, flatbed scanners, and computer gaming consoles. All of these are essentially powerful portable computers with imaging capabilities, and all have the potential to be converted to low-cost, portable optical imaging platforms for mobile POCT.

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Moreover, many biological assays and clinical tests e. Examples of such devices include readers for lateral flow immuno-chromatographic assays [ 50 ], fluorescence detectors [ 515253 ], wide-field fluorescent microscopes [ 54 ], lens-free microscopes [ 55 Ame CU-68 Webcam, capillary array for immunodetection [ 56 ], and fluorescent imaging cytometers [ 57 ].

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More recently, a smart phone-based surface plasmon resonance biosensor has been developed [ 58 ]. Applications for Mobile Optical Detectors Numerous biosensing modalities have been applied for mobile devices including various microscopy technologies [ 195960616263 ], fluorescence biosensors [ 616465666768697071 ], spectroscopy [ Ame CU-68 Webcam7273 ], diffraction [ 7475767778 ], colorimetric biosensors [ 798081828384858687888990919293949596979899 ], chemiluminescence detection [, ], electrochemiluminescence detection [ 23], SPR biosensors [Ame CU-68 Webcam, electrochemical biosensors [ 23,, ], and potentiometric biosensors [].

One of the most commonly used immunological assays is the Enzyme-Linked Immunosorbent Assay ELISA [], Ame CU-68 Webcam designed as an optical detection laboratory test to replace the radioimmunoassay technique.

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Many portable immunology-based diagnostic devices are based on CCD detection systems combined with LOC [ 29434547, ], which have sensitivities comparable Ame CU-68 Webcam laboratory ELISA plate readers for many applications, including microchip ELISA-based detection of the ovarian cancer HE4 biomarker in Ame CU-68 Webcam [ ]. A smartphone with a CMOS detector has been used as a portable ELISA detector [ 76 ], utilizing the integrated phone camera as a spectrometer for detection of IL-6, a protein used diagnostically for several types of cancer, as well as the Ara h 1 protein, one of the principle peanut allergens.

Mobile optical Ame CU-68 Webcam combined with LOC technologies have also been developed for immunodetection of infectious diseases for POCT, including a smartphone dongle combined with microfluidics [ ], a smartphone-based detector used for protein microarray-based fluorescence immunoassay for detection of multiple biomarkers in milk [ ], and an ELISA well plate reader [ ].

A simple CCD-based detector combined with LOC was developed for immunological detection of microbial toxins, including the detection of staphylococcal enterotoxins utilizing densitometry []. This system was also automated for high throughput toxin detection [ ], including fluorescent detection of toxin activity for botulinum neurotoxin A BoNT-A [ 294748 ] and analysis of Shiga toxin 2 Stx2 activity [ 49 ].

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Such technologies are particularly useful in resource-poor settings [ 2930 ]. In recent years several low-cost portable flow cytometers based on webcams have been described [,]. Flow cytometry is another application using optical detection and LOC technology, where high-powered optics are also necessary to achieve microscopic imaging that enables detection of individual cells. In flow cytometry, cell components are fluorescently labeled, separated from each other in a flow field, and then excited by a laser to emit light from the fluorescence label.

There are many clinical applications for flow cytometry, including monitoring HIV infection through Ame CU-68 Webcam T-cell counting, Ame CU-68 Webcam and lymphoma phenotyping, detection of minimal residual cancer disease, and diagnosis of immunodeficiency disorders.

Conventional flow cytometers are based on hydrodynamic focusing of the cells to a narrow flow for detection by individual photomultipliers. However, one limitation of narrow flow is a low flow rate due to the high hydrodynamic resistance and pressure constraints of the cell. This ultimately limits the device to small volumes or Ame CU-68 Webcam analysis times, which is not practical for applications such as rare cell detection.

Imaging-based cytometry can also provide multispectral imagery related to the cell morphology Ame CU-68 Webcam ], protein co-localization on cells [ ]. While very mobile and versatile, the flow rate of this system is low e.

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