AIR SRL A34A Driver
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AIR SRL A34A Driver
This screen identified one Ab2 with AIR SRL A34A binding profile very similar to that of E2. When used as an immunogen in mice, this Ab2 induced Ab3 antibodies that recognize the same epitope and the same residues within it as AP In one aspect the polypeptide of the invention comprises a B2.
Suitably the polypeptide of the invention is a Fab fragment of the B2. The inventors have surprisingly discovered that the Fab fragment of the B2.
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In addition, the Fab fragment is smaller and easier to handle. In addition, by removing sequences AIR SRL A34A required for antigen recognition the Fab fragment presents fewer irrelevant sequences to the immune system AIR SRL A34A the recipient, and therefore provides a more efficient antigen for immunisation.
Suitably the polypeptide of the invention may be a single chain variable fragment scFv derived from the B2. This has the advantage of being of the smallest possible size whilst retaining the antigen binding activity. The polypeptide or antibody or antigen binding fragment thereof of the invention may take any of the known forms.
For example, the polypeptide may comprise an IgG. For example, the polypeptide may comprise a F ab' 2. For example, the polypeptide may comprise a Fab'.
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For example, the polypeptide may comprise a Fab. For example, the polypeptide may comprise a Fv. For example, the polypeptide may comprise a rlgG. The production of AIR SRL A34A of these and any other antibody variants is enabled by the amino acid sequences of the variable regions of the B2. For example, B2.
Suitably a conventional expression system such as the 'antibody generation' system which is commercially available from InvivoGen at 5, rue Jean Rodier, F Toulouse, France may be used. This AIR SRL A34A may then be transfected into any suitable host cell. Suitably the host cell is eukaryotic such as mammalian. Following transfection, the AIR SRL A34A cells are incubated to allow expression of the antibody chains.
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These are the collected, for example from the supernatant in which the cells are incubated. Purification of the antibody chains from that supernatant may be carried out.
Purification may be by any known means such as chromatography, for example affinity chromatography e. Thus, when a full IgG is desired, then the expression vector is so chosen so as to express the chains for a full IgG.
If it is desired to produce a Fab fragment from that IgG, then any standard method known in the art such as papain digestion, pepsin digestion or ficin digestion may be used to generate that Fab. Most suitably, papain AIR SRL A34A of IgG is used to generate Fab. Suitably the antibody or antigen binding fragment thereof of the invention may be administered AIR SRL A34A conjunction with, or formulated into a composition with, a carrier that is suitable for use in humans.
Suitably the antibody or antigen binding fragment thereof of the invention may be administered in conjunction with, or formulated into a composition with, an adjuvant that is suitable for use in humans Alum is a most commonly used adjuvant in human vaccination. It is found in numerous vaccines, including diphtheria-tetanus-pertussis, human papillomavirus and hepatitis vaccines. Alum provokes a strong Th2 response.
Suitably the adjuvant comprises Alum. Suitably alum means aluminium hydroxide, such as in the form of a wet gel suspension. The adjuvant suitably induces both Thi and Th2 responses. In particular, reference is made to their guideline on adjuvants in vaccines for human use document, which is incorporated herein by reference. Suitably the AIR SRL A34A or antigen binding AIR SRL A34A thereof of the invention may be administered as, or provided as, a formulation that is suitable for use in humans.
Known carrier proteins include Keyhole Limpet Haemocyanin KLHself assembling carrier proteins such as Ferritin or luminaze synthase. There are numerous carrier proteins that are commonly used in compositions such as human vaccines: As will be apparent to the skilled person, these are vaccines in their own right, against tetanus and diphtheria, respectively. They are also effective as immunogenic carrier proteins for molecules such as bacterial polysaccharides, which on their own are poorly immunogenic.
In principle, any protein molecule that is used in approved human vaccines could be a suitable carrier.
The same principles apply to a suitable adjuvant. There is a limited number of adjuvants approved for human use, although there are a lot of candidate adjuvants and ongoing research into better human adjuvants, especially within the pharmaceutical industry. In principle, AIR SRL A34A adjuvant approved for use in human vaccines could be suitable.